Comet assay to monitor cell line aging

Single Cell Gell Electrophoresis (Comet Assay)

determination of cell death induced by lovastatin on human colon cell line ht29 using the comet assay

background apoptosis or programmed cell death is an essential process for elimination of damaged cells. also, induction of apoptosis is fundamental for treating cancer. screening for agents that induce apoptosis in tumor cells help in the development of novel agents for cancer treatment. numerous studies suggest that the exposure of tumor cells to statins can lead to cell death via two separate...

Cytotoxicity and genotoxicity assessment of Euphorbia hirta in MCF-7 cell line model using comet assay.

OBJECTIVE To evaluate the cytotoxicity and genotoxicity activity of Euphorbia hirta (E. hirta) in MCF-7 cell line model using comet assay. METHODS The cytotoxicity of E. hirta extract was investigated by employing brine shrimp lethality assay and the genotoxicity of E. hirta was assessed by using Comet assay. RESULTS Both toxicity tests exhibited significant toxicity result. In the comet as...

Determination of Cell Death Induced by Lovastatin on Human Colon Cell Line HT29 Using the Comet Assay

BACKGROUND Apoptosis or programmed cell death is an essential process for elimination of damaged cells. Also, induction of apoptosis is fundamental for treating cancer. Screening for agents that induce apoptosis in tumor cells help in the development of novel agents for cancer treatment. Numerous studies suggest that the exposure of tumor cells to statins can lead to cell death via two separate...

Improvement of single-cell gel electrophoresis (SCGE) alkaline comet assay

Although the single-cell gel electrophoresis (SCGE) alkaline comet assay can detect DNA damage quickly and with high sensitivity, it does not work well for cryopreserved sperm of some marine teleosts. Using large yellow croaker Pseudosciaena crocea sperm that were cryopreserved in increasing concentrations (5 to 30%) of DMSO, we made modifications to the classic SCGE method that included using ...